Fig. 3: Effect of the Gα13 R200K mutation identified in patients on actin organisation and cell morphology. | Nature Communications

Fig. 3: Effect of the Gα13 R200K mutation identified in patients on actin organisation and cell morphology.

From: A postzygotic GNA13 variant upregulates the RHOA/ROCK pathway and alters melanocyte function in a mosaic skin hypopigmentation syndrome

Fig. 3

a Immunofluorescence images of Gα13 WT-YFP, Gα13 R200K-YFP or Gα13 Q226L-YFP (green) expressed in B16-F0 cells. The cells were labelled with phalloidin for visualising F-actin (red), and Hoechst for nuclei (blue). The scale bar is 10 µm. b Quantification of F-actin content that corresponds to the intensity of the red signal. Quantification of cell morphology parameters including perimeter (c), circularity (d) and solidity (e). Means +/− SEM are shown from 75, 103 and 92 Gα13 WT, R200K and Q226L expressing B16-F0 cells, respectively. ANOVA tests were performed. **P = 0.0014; ****P < 0.0001. All graphs shown are representative of eight independent experiments.

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