Fig. 2: Sequence design of residues in proximity to the active site.

a Visualization of the AmVHPO model. Two chains of the decamer are shown. The design of surface-exposed residues was simulated in a 10 Å radius of the catalytic residue K428. Designed residues pointing towards the substrate channel are highlighted in violet. The phosphate position was superimposed from 5LPC21. b Conditional probabilities predicted by ProteinMPNN41 for the designed positions in the AmVHPO dodecamer model.