Fig. 3: Mutant screening and aromatic halogenations of MCD (3) and phenol red (5). | Nature Communications

Fig. 3: Mutant screening and aromatic halogenations of MCD (3) and phenol red (5).

From: Unraveling the molecular basis of substrate specificity and halogen activation in vanadium-dependent haloperoxidases

Fig. 3

a Screening of bromination (orange) and chlorination (ocher) activities of relevant R425 mutants using monochlorodimedone (MCD, 3) assay. The serine variant stands out with a ten times higher conversion of MCD (3) than the wild-type for the chlorination reaction. Comparison of b the aromatic bromination and c aromatic chlorination ability over time using phenol red (5) and d 1,3,5-trimethoxybenzene (7, TMB) using the wild-type AmVHPO and the variants R425D and R425S. Data in b), c) and d) are depicted as mean ± SD from 3 independent experiments performed in technical triplicates. Source data are provided as a Source Data file; AmVHPO = vanadium-dependent haloperoxidase from Acaryochloris marina.

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