Fig. 1: AP1-C stains LDs of all stages in live cells.

A–C Live A549 cells were co-stained with Nile Red (1 μM) and coumarin (10 μM) (n = 8) (A) or AP1- C (10 μM) (B), or Bodipy 493/503 (1 μM) and AP1-C (10 μM) (C) for 1 h, followed by confocal imaging. D, E A549 cells were transiently transfected with mc-ACSL3 (D) or GFP-ADRP (E), then treated with AP1-C (10 μM) for 1 h, followed by confocal imaging. F Pearson correlation coefficient analysis of images in (B–E) was calculated by Zen black software (n = 15 for Nile Red, n = 20 for Bodipy 493/503, n = 15 for ACSL3, and n = 15 for ADRP; compared to the ACSL3 group, p = 0.0069 and p = 0.0034 for ADRP and Bodipy, respectively; p < 0.0001 for Nile Red). G A549 cells were transiently co-transfected with mc-ACSL3 and GFP-ADRP (n = 11), then treated with AP1-C (10 μM) for 1 h or 6 h, followed by confocal imaging. Images were finally captured by a Zeiss 880 microscope with a 63x objective lens. The scale bar is 5 μm. The graphs represented data from three independent experiments, The statistical significance of differences was determined by using the unpaired two-tailed student’s t test, and data quantifications were expressed as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001; ns: no significance.