Fig. 5: Itch- or pain-specific neurons are required for scratching or nocifensive behavior, respectively. | Nature Communications

Fig. 5: Itch- or pain-specific neurons are required for scratching or nocifensive behavior, respectively.

From: Processing of pain and itch information by modality-specific neurons within the anterior cingulate cortex in mice

Fig. 5

a Experimental scheme. b Representative images of emGFP and n-mCherry expression among the ACC of total of 68 mice. Scale bar, 200 μm. c, d Scratching behavior (c) and the proportion of hM4Di-expressing neurons (d) (n = 9 mice in F → H sal, n = 10 mice in F → H CNO. c: two-tailed unpaired t-test, t17 = 0.03335, p = 0.9738. d two-tailed unpaired t-test, t17 = 1.491, p = 0.1544). e, f Scratching behavior (e) and the proportion of hM4Di-expressing neurons (f) (n = 8 mice in H → H sal, n = 11 mice in H → H CNO. e: two-tailed unpaired t-test, t17 = 2.160, *p = 0.0454. f two-tailed unpaired t-test, t17 = 0.7668, p = 0.4537). g, h Nocifensive responses (g) and the proportion of hM4Di-expressing neurons (h) (n = 7 mice in H → F sal, n = 7 mice in H → F CNO. g two-way repeated measures ANOVA: effect of time, F(1,12) = 81.58, p < 0.0001; effect of CNO, F(1, 12) = 0.1758, p = 0.6824; interaction effect, F(1, 12) = 0.01248, p = 0.9129. h two-tailed unpaired t-test, t12 = 0.3180 1, p = 0.7559). i, j Nocifensive responses (i) the proportion of hM4Di-expressing neurons (j) (n = 8 mice in F → F sal, n = 8 mice in F → F CNO. i two-way repeated measures ANOVA followed by Bonferroni post-test: effect of time, F(1,14) = 114.6, p < 0.0001; effect of CNO, F(1, 14) = 11.42, p = 0.0045; interaction effect, F(1, 14) = 15.16, p = 0.0016; post-test, ***p < 0.001. j two-tailed unpaired t-test, t14 = 1.148, p = 0.2703). k, l Schematic diagram of an itch (k) and pain (l) on suppression of modality-specific ACC neurons. sal Saline, F Formalin. H Histamine. Data presented as mean ± SEM. Source data are provided as a Source Data file.

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