Fig. 6: Activation of itch- or pain-specific neurons contribute to inducing pruritogen- and algogen-induced anxious states. | Nature Communications

Fig. 6: Activation of itch- or pain-specific neurons contribute to inducing pruritogen- and algogen-induced anxious states.

From: Processing of pain and itch information by modality-specific neurons within the anterior cingulate cortex in mice

Fig. 6

a Experimental scheme. b Representative images showing mCherry expression in the ACC of a total of 39 mice. Scale bar, 100 μm. c–e Scratching bouts (c, d) and freezing-like behavior (e) when neurons previously activated by histamine were activated using CNO (n = 9 mice in sal, n = 12 mice in CNO. c two-way repeated measures ANOVA: effect of time, F(5, 95) = 2.022, p = 0.0825; effect of CNO, F(1, 95) = 1.834, p = 0.1915; interaction effect, F(5, 95) = 1.277, p = 0.2802. d two-tailed unpaired t-test, t19 = 1.354, p = 0.1915. e two-tailed unpaired t-test, t19 = 1.223, p = 0.2361). f–h Nocifensive responses (f, g) and freezing-like behavior (h) when neurons previously activated by formalin were activated using CNO (n = 10 mice in sal, n = 8 mice in CNO. f two-way repeated measures ANOVA: effect of time, F(11, 176) = 1.188, p = 0.2986; effect of CNO, F(1, 176) = 2.029, p = 0.1736; interaction effect, F(11, 176) = 1.533, p = 0.1231. g: two-tailed unpaired t-test, t16 = 1.424, p = 0.1736. h: two-tailed unpaired t-test, t16 = 2.384, *p = 0.0298). i–k Weak histamine-induced scratching bouts (i, j) and freezing-like behavior (k) when neurons previously activated by histamine were activated using CNO (n = 9 mice in sal, n = 12 mice in CNO. i: two-way repeated measures ANOVA followed by Bonferroni post-test: effect of time, F(5, 95) = 11.62, p < 0.0001; effect of CNO, F(1, 95) = 4.592, p = 0.0453; interaction effect, F(5, 95) = 4.093, p = 0.0021; post-test, ***p < 0.001. j: two-tailed unpaired t-test, t19 = 2.143, *p = 0.0453. k: two-tailed unpaired t-test, t19 = 3.368, **p = 0.0032). l–n, Weak formalin-induced nocifensive responses (l, m) freezing-like behavior (n) and when neurons previously activated by formalin were activated using CNO (n = 10 mice in sal, n = 8 mice in CNO. l two-way repeated measures ANOVA: effect of time, F(11, 176) = 1.884, p = 0.0442; effect of CNO, F(1, 176) = 0.006581, p = 0.9364; interaction effect, F(11, 176) = 0.5102, p = 0.8948. m two-tailed unpaired t-test, t16 = 0.08112, p = 0.9364. n two-tailed unpaired t-test; two-tailed unpaired t-test, t16 = 3.739, **p = 0.0018). sal Saline, H Histamine, F Formalin. Data presented as mean ± SEM. Source data are provided as a Source Data file.

Back to article page