Fig. 2: Chloride concentration drives ethanol valence switch.

a Ethanol chemotaxis responses of wild-type animals to different ethanol concentrations on assay plates containing either 25 mM or 75 mM NaCl. b Chemotaxis responses of wild-type animals to H2O or ethanol across different assay settings, as illustrated on the right. c Chemotaxis responses of wild-type animals to different alcohols or volatile organic compounds. Ethanol chemotaxis responses of wild-type animals on assay plates supplemented with different concentrations of mannitol (d), potassium phosphate (e), sodium phosphate (f), or potassium chloride (g). Statistics were analyzed by two-way ANOVA with Tukey’s multiple comparisons test (a) or one-way ANOVA with Tukey’s multiple comparisons test (b, d, e, f, g). ****p < 0.0001, n.s. statistically not significant. From left to right, P is 0.9345, 0.2836, 0.9888, and 0.0569 (a), <0.0001, 0.9762, 0.201, <0.0001, 0.1964, and 0.9979 (b). Data represent >3 biological replicates performed on different days, with animal numbers shown in parenthesis. Data are shown as means ± SEM. Source data are provided as a Source Data file.