Fig. 2: Functional rescaling of cortical barrel field territories in embWPC mice.
From: A prenatal window for enhancing spatial resolution of cortical barrel maps
a Schema representing the experimental paradigm. b Left, cortical evoked responses (GCaMP6f) to whisker pad (magenta) and upper lip (cyan) stimulations in control (ipsilateral) and whisker pad cauterized (WPC, contralateral) sides in embWPC mice at E18. Control PMBSF and ALBSF territories were translated to the WPC side for direct comparison and labeled as “PMBSF” and “ALBSF”. Right, reslice of the yellow line in the left, representing responses elicited in PMBSF and ALBSF primordia by whisker pad stimulation (wps) or upper lip stimulations (uls). The white horizontal line delineates the boundaries between PMBSF and ALBSF. The vertical dashed line divides the wps from the uls over time. The arrows label the stimulation times for the whisker pad (magenta) and upper lip (cyan). c Quantification of the PMBSF and ALBSF active fraction to wps or uls (n = 7 ctrl, n = 6 WPC). d Left, cortical evoked responses (GCaMP6f) to whisker pad (magenta) and upper lip (cyan) stimulations in control (ipsilateral) and WPC (contralateral) sides in embWPC at P4. Right, reslice of the yellow line in the left, representing responses elicited in PMBSF or ALBSF by wps or uls. The white horizontal line delineates the boundaries between PMBSF and ALBSF. The vertical dashed line divides the wps from the uls over time. The arrows label the stimulation times for the whisker pad (magenta) and upper lip (cyan). e Quantification of the PMBSF and ALBSF active fraction to wps and uls (n = 6 ctrl side, n = 6 WPC side). f Quantification of the percentage of overlap responses between the PMBSF and ALBSF territories at E18 and P4 (n = 9 ctrl E18, n = 6 embWPC E18, n = 9 ctrl P4, n = 6 embWPC P4). g Schema illustrating the results. Dashed lines delineate PMBSF and ALBSF putative evoked territories at E18. Source data are provided as a Source data file. S1, primary somatosensory cortex; GCaMP6f, calmodulin-based genetically encoded fluorescent calcium indicator 6–fast. Scale bars, 1000 μm. Bar graphs show the means ± SEM. ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001.
