Fig. 3: ALBSF rescaling is independent of thalamic patterned activity.
From: A prenatal window for enhancing spatial resolution of cortical barrel maps
a Coronal sections from the Krox20-zsgreen::R2-mCherry double transgenic mouse showing Krox20-labeled axons (magenta, vPrV) and R2-labeled axons (cyan, dPrV) at the VPM at E16. b Quantification of the percentage of overlap of labeled axons (n = 6). c Maximal projection of ex vivo spontaneous calcium activity in the prospective VPM sub-regions (wpVPM and ulVPM) in acute slices at E16. Raster plots of 5 min. The arrows label the calcium waves of synchronous activity. d Quantification of the frequency of wave activity in the VPM (n = 11 ctrl, n = 8 embWPC). e Upper panels, coronal sections of vGlut2 staining (green) in the wpVPM and ulVPM of embWPC and embWPC-ThKir mouse (Kir-mCherry positive) at P3. Lower panels, surface view of TCA-GFP+ terminals in the PMBSF and ALBSF in embWPC and embWPC-ThKir mice at P8. The arrows label the limits between wpVPM and ulVPM in the thalamus, and PMBSF and ALBSF in the cortex. f Quantification of the data (Thalamus, n = 5 ctrl, n = 5 embWPC, n = 6 embWPC-ThKir; Cortex, n = 10 ctrl, n = 13 embWPC, n = 7 embWPC-ThKir). g Upper panels, DiI and DiA crystal placements in the PMBSF and ALBSF, respectively. Lower panels, backlabelled cells at the thalamus show shifted positions within the ulVPM thalamic area in both embWPC and embWPC-ThKir mice at P8. h Quantification of the position of backlabelled cells with respect to the distance to the dLG nucleus. The gray horizontal dashed line in the graph represents the separation between wpVPM and ulVPM (n = 6 embWPC, n = 6 embWPC-ThKir). Source data are provided as a Source data file. Scale bars, a 100 μm; c, g (bottom) 200 μm; e (top) 250 μm, e (bottom), g (top) 500 μm. Boxplots show the medians with the interquartile range (box) and range (whiskers). Bar graphs show the means ± SEM. ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001.
