Fig. 9: Type I collagen production by epidermal cells by various animal species.
From: Keratinocyte-driven dermal collagen formation in the axolotl skin
a–c Pulse-chase experiments with Danio rerio. a DAF and CHP-Cy3 signals at the epithermal side. a1 CHP-Cy3, a2 DAF, a3 The marge image in (a1, a2). b, b’ Orthogonal view from the reconstituted confocal microscopic images. c The histogram of relative sum of brightness. d–g Hamburger–Hamilton stage 26 embryo in Gallus gallus domestics d Col1a2 gene expression pattern in the whole body section, e in the forelimb bud. f High magnification view in E. f1 Col1a2 gene expression pattern. f2 Immunofluorescence of COL4. f3 The Merge image of (c1, c2). f1–f3 are the flipped images of the boxed region in (e). g1 The negative control in the ectoderm. g2 High magnification view in (d1). h–k Stage of E13.5 in Mus musculus. h Col1a2 gene expression pattern in lower body section, i in the hindlimb bud. j High magnification view in (f). j1 Col1a2 gene expression pattern. j2 Immunofluorescence of COL4. j3 Merge images of 1 and 2. j1–j3 are the flipped images of the boxed region in (i). k1 The negative control in backbone. k2 High magnification view in (k1). The d–k (exclude f2, j2) used pseudo-coloring. In f3 and j3, epi. and mes. indicate epidermis and mesenchyme. The scale bars in a, f1, g2, j1, and k2 are 10 μm, d, h are 500 μm, e, i are 100 μm, g1 and k1 are 100 μm. The results were confirmed in five biologically independent samples and experiments. Source data are provided as a Source Data file.
