Fig. 2: Expression of NS2 complicates simple length-dependent replication kinetics. | Nature Communications

Fig. 2: Expression of NS2 complicates simple length-dependent replication kinetics.

From: Analysis of NS2-dependent effects on influenza PB1 segment extends replication requirements beyond the canonical promoter

Fig. 2

a, b Barcoded 200, 400, 800, and 1600nt variants of each individual segment bearing equal sequence length derived from 5’ and 3’ ends and lacking canonical start codons were generated and co-transfected into HEK293T cells along with the minimal replication machinery with, or without, NS2. The relative frequency of vRNA of each variant was measured by qPCR at 24 hours post-transfection, with 1600nt variants serving as a comparison. Asterisks indicate conditions significantly impacted by the expression of NS2, two-sample two-tailed t-test with a within-panel Benjamini-Hochberg corrected FDR < 0.05. n = 3 for (a), n = 4 for (b), individual replicates and mean displayed. Source data are provided as a Source Data file.

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