Fig. 5: FASN enhances mutp53 GOF in promoting anchorage-independent growth (AIG) and tumorigenesis.

a FASN KO displayed a more pronounced inhibitory effect on AIG in SK-BR3 cells compared with SK-BR3 with mutp53 KO. Left: Representative images of AIG of cells. Scale bar: 200 μm. b FASN knockdown displayed a more pronounced inhibitory effect on AIG in MDA-MB468 and LS1034 cells compared with corresponding cells with mutp53 knockdown. c FASN knockdown displayed a more pronounced inhibitory effect on AIG in p53^R248W/- HCT116 and E1A/Ras-transformed p53^R172H/R172H MEF cells compared with their corresponding p53^+/+ or p53^-/- cells. d Ectopic FASN expression displayed a more pronounced promoting effect on AIG of SK-BR3 and p53^R248W/- HCT116 cells compared with SK-BR3 cells with mutp53 KO and p53^+/+ or p53^-/- HCT116 cells, respectively. e FASN KO displayed a more pronounced inhibitory effect on the growth of orthotopic tumors formed by SK-BR3 cells compared with tumors formed by SK-BR3 cells with mutp53 KO. Left: The image of collected tumors. Scale bar: 10 mm. Right: The weights of collected tumors. FASN KO reduced mutp53 protein levels in SK-BR3 tumors analyzed by Western blot (f) and IHC staining (g) analysis. Scale bar: 40 μm. h FASN knockdown displayed a more pronounced inhibitory effect on the growth of tumors formed by p53^R248W/- HCT116 cells compared with tumors formed by p53^+/+ or p53^-/- HCT116 cells. Left: The image of collected tumors. Scale bar: 10 mm. i FASN knockdown reduced mutp53 protein levels in p53^R248W/- HCT116 tumors but not wtp53 protein levels in p53^+/+ HCT116 tumors. In f, i, similar results were observed in samples from at least 6 mice/group, and results from 3 mice/group are presented. Data represent mean ± SD (n = 6 independent experiments in a–d; n = 10 mice/group in e, h). In d, two-tailed unpaired Student’s t-test. In a, b, c, e, and h, one-way ANOVA followed by Dunnett’s test. Source data are provided as a Source Data file.