Fig. 4: SAR247799 protects the endothelial barrier in human colon crypts and human umbilical vein endothelial cells (HUVEC). | Nature Communications

Fig. 4: SAR247799 protects the endothelial barrier in human colon crypts and human umbilical vein endothelial cells (HUVEC).

From: S1PR1-biased activation drives the resolution of endothelial dysfunction-associated inflammatory diseases by maintaining endothelial integrity

Fig. 4

a Representation IF staining Icam (cyan), Vcam1 (orange), E-selectin (light yellow) CD31 (red) of induced human ulcerative colitis organoid (iHUCO) generation protocol followed by TNF-α administration (Negative group) with SAR247799 (SAR group) and FTY720 (FTY720 group) treatment (n = 3); Scale bar, 200 μM. b Summary of the count of DEGs identified in all pairwise comparisons of differential expression analysis using Gfold with RNA-seq data, with each group containing one sample. c, d Box-plot illustrating GSVA scores for pathways related to endothelial functions or related to immune. Each point represents the GSVA score of a pathway. The definition of the box plots can be found in the ‘Methods’ section. e, f The KEGG enrichment results. The figures depict pathways that ranked among the top 10 in at least one set of pairwise comparisons. g, qRT-PCR analysis of S1PR1 expression levels in HUVEC cells after transfection with S1PR1 shRNA and overexpression S1PR1 plasmid (n = 3). h RT-PCR analysis of endothelial and chemokine markers in knockdown and overexpression S1PR1 HUVEC cells (n = 3). Data are presented as mean ± SEM; Statistical analysis: GFOLD (b), two-sided Student’s t test (c and d, g, and h), and Hypergeometric Test (e and f). All biological experiments were repeated three times and yielded consistent results. Source data are provided as a Source Data file.

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