Fig. 5: O-demethylation of S-lignin aromatics by SyoA using the peroxide shunt.
From: Structural insights into S-lignin O-demethylation via a rare class of heme peroxygenase enzymes
Schemes showing the major products produced during the O-demethylation of syringol, 4-methylsyringol and syringaldehyde by SyoA. Reactions were carried out using 1 µM SyoA in 50 mM Tris pH 7.5, 10 mM H2O2; and 0.5 mM syringol, 0.5 mM syringaldehyde or 2 mM 4-methylsyringol at 30 °C for 60 min prior to quenching with catalase. Products were analyzed and the total concentration of the demethylated aromatic product measured. Error bars indicate ± 1 standard deviation from the mean of 3 replicates (black dots) from distinct samples including: 3-methoxycatechol 155, 125, 146 μM; 3-methoxy-5-methylbenzene-1,2-diol 802, 822, 762 μM; and 5-hydroxyvanillin 332, 314, 279 μM.
