Fig. 10: Evolutionary History of GH101s.
From: Dual functional POGases from bacteria encompassing broader O-glycanase and adhesin activities
a Phylogeny of Endo-α-N-acetylgalactosaminidases as a marker gene with the WAG + F + I + G4 substitution model in IQ-TREE. Maximum Likelihood Phylogenetic tree showing that POGases are all evolutionarily related and are expressed in Actinomycetota. Other GH101 domains containing GHs are evolutionarily distinct and are expressed in Firmicutes. b The evolutionary history of the species expressing the GH101s was also generated but using 16s rRNA nucleotide sequences from Actinomycetota and Firmicutes with the SYM + I + G4 substitution model in IQ-TREE. The evolutionary history of the proteins matches that of the species in which it’s expressed, indicating the proteins evolved with the species as a niche was found.
