Fig. 4: MS analysis of CD24 and MUC5AC O-glycopeptides after POGase Treatment.
From: Dual functional POGases from bacteria encompassing broader O-glycanase and adhesin activities
a Schematic illustration of analytical strategy: released O-glycans and peptides from glycopeptides by POGase AS were proceeded with permethylation for O-glycan analysis on MALDI-TOF and LC-MS/MS analysis, respectively. b Peptides from glycopeptides carrying Core 1: spectra of CD24 glycopeptides and MUC5AC glycopeptides carrying two Core 1 (CD24-T2 and MUC5AC-T2) and their corresponding peptides after POGase AS treatment were shown. c Mass spectra of digested CD24 glycopeptides carrying sialylCore 1: MS1 of α2,3sialylCore 1 CD24 before and after POGase AS treatment were shown. d Mass spectra of digested MUC5AC carrying α2,3sialylCore 1: MS1 of α2,3sialylCore 1 MUC5AC before and after POGase AS treatment were shown. e Mass spectra of digested MUC5AC carrying α2,3sialylCore 2: MS1 of α2,3sialylCore 2 MUC5AC before and after POGase AS treatment were shown. In all cases, the unmodified peptide was present after POGase release. Source data are provided as a Source Data file.
