Fig. 2: Migration behavior of HUVECs on the surface with different topographies.
From: Drop-shaped microgrooves guide unidirectional cell migration for enhanced endothelialization
a Cell trajectories (n = 11–12 cells) after 2 h of migration on the flat surface or on different microgrooves. Statistical analysis of the b directionality ratio and c speed of cell migration on different surfaces (n = 11 cells). d Comparative analysis of cell migration directionality on different surfaces. e Cell migration process in drop-shaped microgrooves. Red: CellTrackerTM orange. Scale bars are 20 μm. f Staining of keratin (green) and nuclei (blue) of HUVECs migrated on different surfaces. g Quantitative analysis of keratin intensity on different surfaces. The red line indicates the nuclei area. The black arrow represented the RhoA polarization signal. h Staining of RhoA (green) and nuclei (blue) of HUVECs migrated on different surfaces and the quantitative analysis of RhoA fluorescence intensity changes. RhoA activity was detected using fluorescence resonance energy transfer (FRET) biosensor. The black arrow represented the RhoA polarization signal. The data were representative of three independent experiments and expressed as the mean ± standard deviation (SD). Data are presented as means ± SD. Significance determined by unpaired two-tailed t-test (c).
