Fig. 6: Histologic examinations of the LAA occluders implanted in the canine LAA.

H&E staining, Masson staining, vWF staining and αSMA staining of neo-tissue on flat and Drop-s surface at 7 days (a) and 14 days (b). Quantitative results of collagen density (c), αSMA-positive area (d), and vWF-positive area (e) at 7 days and 14 days (Five measurements of collagen density and three measurements of positive area were taken for 5 different fields of view from 3 different regions of tissue). f Images of RhoA staining (green) and DAPI (blue) of the neo-tissue at 7 days and 14 days. g Quantitative results of RhoA-positive area (Five measurements of RhoA-positive area were taken for 5 different fields of view from 3 different regions of tissue). Quantitative analysis of collagen orientation (h) and cell orientation (i) results based on tissue section images. The data were expressed as the mean ± SD. Significance determined by unpaired two-tailed t-test and p > 0.05 was considered as not significant.