Fig. 3: Effect of PAM 5′ terminal nucleotides on Cas12a cleavage to target sequences. | Nature Communications

Fig. 3: Effect of PAM 5′ terminal nucleotides on Cas12a cleavage to target sequences.

From: Highly parallel profiling of the activities and specificities of Cas12a variants in human cells

Fig. 3

Indel frequencies for different PAM sequences for enAsCas12a-HF1, AsCas12a Ultra, AsCas12aRR, AsCas12aRVR (a) and LbCas12aRR, LbCas12aRVRR, LbCas12a, LbCas12aRVR, mut2C-W, mut2C-WF (b). V = A/C/G; D = A/G/T; B = C/G/T. The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences (n) are as follows: n = 359 for DTTCV, 124 for CTTCV, 366 for DTGTV, 127 for CTGTV, 374 for DTGCV, 126 for CTGCV, 372 for DTCCV, 125 for CTCCV, 237 for DTATV, 125 for CTATV, 363 for DTACV, 118 for CTACV, 372 for DGTTV, 122 for CGTTV, 367 for DGTCV, 125 for CGTCV, 363 for DCTTV, 122 for CCTTV, 369 for DCTCV, 120 for CCTCV, 374 for DCCCV, 125 for CCCCV, 358 for DATTV, 121 for CATTV (enAsCas12aHF1); n = 374 for DTCTV, 127 for CTCTV (AsCas12aUltra); n = 374 for DCCCV, 125 for CCCCV, 367 for DGTCV, 125 for CGTCV, 373 for DTCCV, 125 for CTCCV, 358 for DTTCV, 122 for CTTCV (AsCas12aRR); n = 351 for DTATV, 120 for CTATV (AsCas12aRVR); n = 380 for VCCCV, 127 for TCCCV, n = 377 for VCTCV, 122 for TCTCV, 369 for VTTAV, 126 for TTTAV (LbCas12aRR); n = 365 for BTACV, 123 for ATACV (LbCas12aRVRR); n = 374 for VCTTV, 124 for TCTTV (LbCas12a); n = 376 for VCTCV, 124 for TCTCV, n = 365 for VCTTV, 120 for TCTTV, 362 for BTACV, 125 for ATACV, 353 for BTATV, 117 for ATATV, 375 for BTCCV, 127 for ATCCV, 358 for BTGCV, 124 for ATGCV, 368 for BTTCV, 125 for ATTCV (LbCas12aRVR); 365 for VCTTV, 121 for TCTTV (mu2c-W); n = 367 for VCTTV, 123 for TCTTV (mu2c-WF); paired t-test, two tailed. Source data are provided as a Source Data file.

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