Fig. 6: Systematically comparing ABE8e and GS-ABE8e gene editing performance on human genomic loci in HEK293T cells.

a Workflow for testing GS-ABE8e in HEK293T cells. b Evaluation of the A-to-G editing efficiencies of ABE8e and GS-ABE8e at 11 representative endogenous genomic sites in HEK293T cells. Data are presented as mean ±s.d. (n = 3). c Indel formation in HEK293T cells treated as described in (b). Data are presented as mean ±s.d. (n = 3). d Comparison of indels induced by ABE8e and GS-ABE8e at 12 target sites including 11 sites in (c) and EMX1 site in Fig. 4i. Each data point represents the average indel frequency at each site calculated from 3 independent experiments. P-values were determined by two-tailed student’s t-test. e Frequencies of A-to-G editing by ABE8e or GS-ABE8e editor across the protospacer positions 1-20 from (b). f Cas9-dependent DNA off-target analysis comparing ABE8e and GS-ABE8e at ABE site 2. g Cas9-dependent DNA off-target analysis comparing ABE8e and GS-ABE8e at EMX1 site. h, i On-target:off-target editing ratios for two sites in (f) and (g). For all plots, bars represent mean values, and error bars represent the s.d. of three independent biological replicates. j Indel formation at two off-target sites in (f). k Indel formation at five off-target sites in (g). Source data are provided as a Source Data file.