Fig. 2: Methanogenic and ETC gene expression patterns match their functional order.
a–c Cumulative (blue) and rate (red) curves (mean ± s.e.m.) for cell growth (a), CH4 production (b), and methyl consumption (c) over time under the optimal growth condition, respectively. Data from three biological replicates. Shadings indicate s.e.m. d Representative image of LMO-1 cells grown in the optimal condition (15 hpi), with seven methanogenic enzyme genes and five ETC genes labeled, each represented by a unique color. Scale bar, 2 μm. Experiments were repeated twice with similar results. e, f Temporal expression patterns (mean ± s.e.m.) of methanogenic genes (e) and ETC genes (f), respectively. Left: simplified pathway diagrams, with inactive components in gray. Middle: For each gene, mRNA level (y-axis) is plotted as a function of time. Red arrows indicate expression peaks. Log-phase data were from two biological replicates with ≥10 fields of view (FOVs). Shadings indicate s.e.m. calculated from the means of each field of view. n = 8787, 15,150, 14,001, 15,835, 23,096, 4707, 5346, 2568, 3575, 3017, 3009, and 3054 cells at time points from early to late. Right: smHCR-FISH signals for each gene, corresponding to the white box in (d). Scale bars, 2 μm. Source data are provided as a Source Data file.
