Fig. 4: Single-cell analysis reveals dynamic clusters of methanogenic/ETC gene expression. | Nature Communications

Fig. 4: Single-cell analysis reveals dynamic clusters of methanogenic/ETC gene expression.

From: Uncovering dynamic transcriptional regulation of methanogenesis via single-cell imaging of archaeal gene expression

Fig. 4

a, b UMAP analysis of methanogenic and ETC gene expression from all time points under optimal (−Fe(III), a) and ferrihydrite-amended (+Fe(III), b) conditions. Each dot represents a single cell. Identified clusters are shown in different colors, with a Sankey diagram relating clusters in two growth conditions. Each cluster’s gene expression profile is displayed on the right. c, d Cell distribution in each cluster as a function of time for optimal (−Fe(III), c) and ferrihydrite-amended (+Fe(III), d) conditions. a, c n = 32,885 cells from 11 time points. b, d n = 6408 cells from nine time points. Source data are provided as a Source Data file.

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