Fig. 2: faf genetically interacts with ft.

a Schematic representation of the faf locus. mRNA transcripts encoded in the faf locus are represented (boxes depict exons, while introns are shown as black lines. Coding sequences are shown as blue boxes, and UTRs are represented as grey boxes). Mutant alleles used are mapped onto the faf locus. faf ^B3 corresponds to a nonsense mutation leading to a truncated Faf protein (Q⁷¹ > STOP). The faf ^BX4 lesion is the site of an inversion breakpoint. faf ^F08 is a point mutation in the DUB catalytic domain (H¹⁹⁸⁶ > Y). faf ^BX3 corresponds to a 15 bp deletion in the vicinity of an exon-intron boundary. b–g Loss of one copy of faf enhances the overgrowth of ft mutants. Shown are tiled confocal images of wing imaginal discs. Compared to control L3 imaginal discs (w^iso, b), the trans-heterozygous combination of ft^G-rv and ft⁸ leads to a dramatic increase in tissue size (c), which is further enhanced by the presence of a faf mutant allele (faf ^F08 (d), faf ^BX4 (e), faf ^B3 (f), faf ^BX3 (g)). h Quantification of relative wing imaginal disc size. Data are represented as % of the average imaginal wing disc area of controls (w^iso, which was set to 100% and indicated by the black dashed line). Red dashed line indicates average wing disc size of ft trans-heterozygous mutants. Data are shown as average ± standard deviation, with all data points depicted. (n = 11, 17, 20, 8, 13 and 9). Significance was assessed using Brown-Forsythe and Welch one-way ANOVA analyses comparing all genotypes to the respective control (w^iso or ft^G-rv/ft⁸; black or blue asterisks, respectively), with Dunnett’s multiple comparisons test. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Scale bar: 100 µm.