Fig. 3: faf genetically interacts with ds, Dlish and Fbxl7 in the regulation of wing tissue growth.

a–o Modulation of Faf expression levels affects the tissue growth phenotypes of ds- (b–f), Dlish^RNAi- (g–k) and Fbxl7-expressing flies (l–o). Shown are adult wings from flies raised at 25°C expressing the indicated transgenes in the wing pouch under the control of nub-Gal4 (nub > ). Compared to control adult wings (a), wings expressing ds displayed significant tissue undergrowth (b), which was partially rescued by simultaneous depletion of faf (c, d and e) and enhanced by faf over-expression (f). RNAi-mediated depletion of Dlish (g) caused a mild reduction in wing size, which was partially suppressed by co-depletion of faf (h), and enhanced by faf over-expression (i). Expression of ft resulted in an enhancement of the undergrowth of Dlish^RNAi wings (j), and this was in part prevented by faf^RNAi (k). Fbxl7 over-expression caused reduced wing size compared to controls (l) and this was not significantly affected by faf^RNAi (m) or ft expression (n), but this was partially suppressed when ft was combined with faf^RNAi (o). p–r Quantification of relative adult wing sizes in genetic interactions with ds (p), Dlish (q) or Fbxl7 (r). Data are represented as % of the average wing area of control wings (nub > GFP, which were set as 100%). Data are shown as average ± standard deviation, with all data points represented. (n = 23, 25, 18, 30, 30 and 18 for (p); n = 20, 28, 26, 19, 29 and 28 for (q); and n = 23, 28, 30, 25 and 27 for (r). Black dashed lines represent average size of controls (100%) whilst red dashed lines indicate average size of adult wings from flies expressing ds, Dlish^RNAi or Fbxl7 under the control of nub-Gal4. Significance was assessed using a one-way ANOVA comparing all genotypes to their respective controls (nub > GFP, nub > GFP + UAS-Ds, nub > GFP+Dlish^RNAi or nub > GFP + UAS-Fbxl7; black, green, brown or blue asterisks, respectively), with Dunnett’s multiple comparisons test. **, p < 0.01; ***, p < 0.001. n.s. non-significant. Scale bar: 500 µm.