Fig. 6: The role of Faf in modulating Ft function is dependent on its DUB activity.

a–d Faf regulates Yki target gene expression in a DUB-dependent manner. XY confocal sections of third instar wing imaginal discs carrying ex-lacZ (a–d, shown in red in a’–d’ merged images), in which en-Gal4 was used to drive expression of GFP (a), faf^isoC (b), faf^WT (c) or faf^CD (d). GFP (green in a’-d’ merged images) indicates posterior compartment where transgenes are expressed. DAPI (blue) stains nuclei. Dashed lines indicate anterior-posterior compartment boundary. e Quantification of ex-lacZ expression levels. Shown are the posterior/anterior (P/A) ex-lacZ ratios for the different genotypes analysed. Data are shown as average ± standard deviation, with all data points represented. (n = 9, 13, 20 and 18). Significance was assessed using a one-way ANOVA comparing all genotypes to UAS-GFP, with Dunnett’s multiple comparisons test. *, p < 0.05; ***, p < 0.001; ns, non-significant. Scale bar: 50 µm. f–t Effect of Faf on Ft-mediated regulation of growth is DUB-dependent. Shown are adult wings from flies raised at 25°C expressing transgenes under nub-Gal4 control. (j), (o), (t) Quantification of effect of Faf on tissue growth. Shown are relative adult wing sizes from flies expressing the indicated transgenes under nub-Gal4 control. Data are represented as % of the average wing area of controls (nub > GFP, set to 100%). Data are shown as average ± standard deviation, with all data points depicted. (n = 22, 18, 30, 26 for j; n = 22, 21, 22, 29, 32 for o; and n = 22, 19, 19, 29 and 25 for t). Significance was assessed using a one-way ANOVA analysis comparing all genotypes to nub > GFP (black asterisks), to nub > UAS-ft (green asterisks), or to nub > UAS-ft^RNAi (blue asterisks) with Dunnett’s multiple comparisons test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, non-significant. Scale bar: 500 µm.