Fig. 6: Architecture and phylogenetic relationships of genes primarily expressed in Prisilkin-like+ cells.

A Region on chromosome 2 of Hydractinia depicting genomic location of Prisilkin-39 and one annotated Shematrin-like gene. B Dot plot showing individual expression levels of genes specific to the Prisilkin-like+ cluster in the same genomic location ranges of (A). C Signal peptide regions (N, H, and C-terminal regions) and glycine repetitions (singlet, duplet, triplet, quadruplet) across complete amino acid sequences of all genes in (B). (tomato red; C, lime green; N, gold; H, cornflower blue; singlets, indian red; duplets, light grey; triplets, plum; quadruplets). D Phylogeny of cnidaria adapted from De Biasse et al. depicting number of annotated proteomes in NCBI (empty blocks), number of annotated Shematrin-like (Lilac blocks) and Prisilkin-39 proteins (Turquoise blocks). Clade silhouettes were obtained from www.phylopic.org and are available under public domain, except for the Octocorallia silhouette (credit to Qiang Ou, CC-BY 3.0 https://creativecommons.org/licenses/by/3.0/). E Signal peptide regions (N, H, and C-terminal regions) and glycine repetitions (singlet, duplet, triplet, quadruplet) across complete amino acid sequences of all Shematrin-like and Prisilkin-39-like annotated proteins in cnidarians. F Signal peptide regions (N, H and C-terminal regions) and glycine repetitions (singlet, duplet, triplet, quadruplet) across complete amino acid sequences of a selection of Shematrin-like and Prisilkin-39-like annotated proteins in molluscs. G Dotplots showing individual expression levels of genes found Hydractinia related to biomineralization pathways. H Unrooted phylogenies of genes annotated as Alpha Carbonic Anhydrase. In each tree, animal taxa are highlighted with legend provided accordingly. All sequences belonging to Hydractinia symbiologicarpus are highlighted in bold. Leaves on trees include species names, accession numbers if obtained from NCBI, and sequence annotation. Ultrafast bootstrap values are reported on nodes with red dots if > = 95. I Maximum projection from a confocal stack of Prisilkin-39 SABER-FISH in a 3-day post metamorphosis, substrate adhered, feeding polyp, imaged from the aboral pole. *Indicates oral pole, arrows indicate direction of outgrowth of stolon, scale bar = 80 μm. J Selection of dotplots showing scored expression of one-to-one ortholog genes present in annotated clusters of Stylophora pistillata with minimum fold change of 1 (Calicoblasts, Neurone 6 and Cnidocytes).