Fig. 2: The GAS-specific IgG antigenome across healthy and sepsis individuals.

a Rank correlation network of the 72 GAS antigens identified across different individuals. Nodes represent each identified GAS antigen and the distance between the nodes is defined by edges encoding Kendall tau coefficients for each pairwise comparison. The color of the edges reflects positive (red) or negative (blue) correlation coefficients. To be considered part of the antigenome, the proteins were required to be present in at least two out of three biological replicates, and identified by at least two quantifiable peptides, having at least a two-fold enrichment over the negative control (Xolair, a commercial anti-IgE monoclonal). Pearson correlation clustering of the log2 intensity of the antigens in (b) Cluster A, (c) Cluster B, and (d) the 11 common antigens across healthy and sepsis individuals. e Sequence conservation plots of the 72 antigens based on the analysis of gap frequency, entropy, and gene carriage of each protein across 2275 GAS genomes (left), and zoom in plot of the antigens excluding M1 (right). Residues with high conservation have low entropy, whereas residues with low conservation have high entropy. Gaps indicate insertion and deletion in sequences.