Fig. 3: Comparative analysis of the GAS and SD antigenome.

a 72 GAS antigens mapped to homologous sequences with >50% identity across 268 different SD genomes. The 11 GAS antigens commonly identified across all individuals (Fig. 2D) are highlighted in red. b Sequence variation plot of the GAS antigen homologues based on the analysis of gap frequency, entropy, and gene carriage of each protein across SD genomes. Residues with high conservation have low entropy whereas residues with low conservation have high entropy. Gaps indicate insertion and deletion in sequences. c Volcano plot displaying the significant SD antigens recognized by IVIG and (d) pooled human plasma (HP). Statistical significance was determined using a both side t-test with an FDR of 0.05 to correct for multiple comparisons. e Venn diagram depicting unique and common GAS and SD antigens recognized by IVIG and HP. f Alluvial plots representing the fold change and sequence homology of antigens significantly enriched from both GAS and SD lysates using IVIG and (g) HP. h Pie chart of antigens significantly enriched from either GAS or SD lysates using IVIG and HP. The area of the pie is the average intensity of triplicates expressed as a percentage of the total intensity of all antigens. i Differential expression of the log2 intensity of unique and common antigens of GAS or SD antigenome across secreted (S), cell wall (CW), and membrane (M) fractions of the GAS and SD lysates. (j) Bar plots representing log2 intensity of SD M protein across the lysates and pulldown using Xolair, IVIG, HP, plasma from healthy (n = 5) and convalescent plasma (n = 5) from sepsis individuals. Bars represent mean values and error bars represent standard deviations. Each sample was analyzed as three independent pulldowns. k Sequence alignment of M proteins from GAS_SF370 and SD_stG62647. Source data are provided as a Source Data file.