Fig. 4: Epitope mapping of GAS antigens. | Nature Communications

Fig. 4: Epitope mapping of GAS antigens.

From: Dissecting the properties of circulating IgG against streptococcal pathogens through a combined systems antigenomics-serology workflow

Fig. 4

a Schematic representation of the epitope extraction workflow (EpXT) to identify epitopes on recombinantly expressed antigens. b Identified peptides (marked red) by EpXT mapped onto the crystal structure of C5AP (left) and their relative intensity (%) is shown on the C5AP cartoon (right). c Deuterium uptake plots for two peptide stretches of C5AP alone (yellow) and when incubated with IVIG (blue). d Identified peptides (marked blue) by HDX-MS (left) and overlapping epitopes identified by both HDX-MS and EpXT mapped onto the crystal structure of C5AP (right). e Heatmap of M1 peptide intensities across IVIG, healthy, and sepsis individuals. f Consensus epitope landscape across all individuals with more than 5% epitope frequency are displayed in the M1 cartoon and the M1 model.

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