Fig. 5: GAS-specific antibodies trigger immune signaling in an antigen- and receptor-specific manner. | Nature Communications

Fig. 5: GAS-specific antibodies trigger immune signaling in an antigen- and receptor-specific manner.

From: Dissecting the properties of circulating IgG against streptococcal pathogens through a combined systems antigenomics-serology workflow

Fig. 5

a FcγRIIa (CD32) and (b) FcγRIIIa (CD16) activity assay of M1, C5AP, and PRGA specific antibodies present in IVIG. c Antibody titers in IVIG against M1, C5AP, and PRGA. d Subclass enrichment profiles of antigen-specific IgG in IVIG. e FcγRIIa (CD32) and (f) FcγRIIIa (CD16) activity assay of mAb25 in IgG1-4 scaffolds. Dotted lines indicate the background values. Statistical significance was assessed by either one-way or two-way ANOVA with correction for multiple comparisons, *p < 0.05. The results are the average of experiments done in triplicates. Bars represent mean values and error bars represent standard deviations. g FcγRIIa (CD32) and (h) FcγRIIIa (CD16) activity assay of M1-specific antibodies across healthy and sepsis individuals. Quantification of M1-specific (i) IgG3, (j) IgG1, (k) IgG2, and (l) IgG4 enriched from plasma derived from healthy (n = 12) and sepsis individuals (n = 7) based on the LFQ intensity of the most intense proteotypic peptide of each IgG subclass. Statistical significance was assessed by a two-sided Mann–Whitney unpaired t-Test, *p < 0.05. The results are the average of experiments done in triplicates and bars represent mean values. Source data are provided as a Source Data file.

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