Fig. 2: High Energy Resolution Fluorescence Detection (HERFD) X-ray Absorption Spectroscopy of Tyr34Phe MnSOD. | Nature Communications

Fig. 2: High Energy Resolution Fluorescence Detection (HERFD) X-ray Absorption Spectroscopy of Tyr34Phe MnSOD.

From: The role of Tyr34 in proton coupled electron transfer and product inhibition of manganese superoxide dismutase

Fig. 2

a The HERFD-XANES region of Tyr34Phe MnSOD treated with potassium dichromate to isolate the Mn3+SOD resting state, sodium dithionite to isolate the Mn2+SOD resting state, and either superoxide or hydrogen peroxide to isolate the product-inhibited state. b Overlay of the Fourier transform of Mn K-edge EXAFS data [k2χ(k)] from superoxide- and peroxide-soaked Tyr34Phe MnSOD with the EXAFs spectrum in k-space shown in the inset. Due to the scattering phase shift, the distance found by the Fourier Transformation (R) is ~0.5 Å shorter than the actual distance and a 0.5 Å correction (α) was implemented. c, d Superoxide- and peroxide-soaked Tyr34Phe MnSOD with the EXAFs spectrum. Colored lines represent experimental data, while the black line is simulated EXAFS spectra fit to the experimental data.

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