Fig. 3: Crystal structure of P domain in complex with glycan-insensitive bnAbs. | Nature Communications

Fig. 3: Crystal structure of P domain in complex with glycan-insensitive bnAbs.

From: Broadly neutralizing antibodies isolated from HEV convalescents confer protective effects in human liver-chimeric mice

Fig. 3

Cartoon view of complex structures of the GT3 P domain (gray) with p60.15 (red; a) and p61.15 scFv (blue; b), which recognize partially overlapping epitopes on the P domain dimer (gray); only the bnAb fragment binding to the right P domain protomer is shown for clarity. The position of the N-linked glycosylation site N3 is indicated. c–f Side-view of the footprints (yellow) of human “glycan-insensitive“ bnAbs on the P domain dimer (gray) in comparison to described murine nAbs 8G12 (PDB 4PLJ;26; e) and 8C11 (PDB 3RKD;27; f). 8G12 is the only antibody that binds at the P domain dimer interface. Sidechain oxygen and nitrogen atoms of N562 are colored in red and blue, respectively, to indicate the position of N3.

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