Fig. 3: In-vivo imaging of transgenic mice expressing Kat2-TGA-Fluc construct.

A Quantification of Luciferase (Fluc), Katushka (Kat2), and Fluc/Kat2 ratio by in-vivo imaging analysis at time points 6, 9, 12, and 15 months in liver, retina, and hindlegs; left graph: Fluc, measured in RLU, shown as mean ± SEM; middle graph: Kat2, measured in RFU, shown as mean ± SEM; right graph: Fluc/Kat2 ratio shown as mean ± SEM. Number of animals tested: for all animals N = 14 for 6 and 9 months, N = 12 for 12 and 15 months; for males N = 6 for 6 and 9 months, N = 5 for 12 and 15 months; for females N = 8 for 6 and 9 months, N = 7 for 12 and 15 months. 6 months values were set as 1. P values were calculated using two-sided Student’s t-test with equal variances. ο P = 0.12, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001, n.s. P > 0.05. Exact P values are present in the Source Data File. B Representative images of wild type (33 F) and transgenic (35 F) female mice for in-vivo analysis of liver, retina and hindlegs at 6 months and 15 months’ time points. (top) Katushka fluorescence, (bottom) Fluc luminescence. Kat2 fluorescence was measured at Ex: 605 nm, Em: 660 nm, Bin: Small, Exposure: 1 s. Fluc luminescence was measured at Bin: Small, Exposure: 5 s. Tissue-specific ROIs (Region-of-Interest) are indicated by the red edging in B; fluorescence (Kat2) and luminescence (Fluc) intensities were measured in equal areas.