Fig. 1: Schematic diagram of preparation and therapeutic mechanism of APOs@atRANBPmiR-124-enginneered MSCs (APOs@atRANBPmiR-124-eMSCs) for AD treatment.
From: Serum-tolerant polymeric complex for stem-cell transfection and neural differentiation
a For preparation, miR-124, APOs, and atRAN were integrated into BP to prepare APOs@atRANBPmiR-124 through electrostatic binding and boron-nitrogen coordination. b The APO corona shells prevented serum-protein adsorption and enhanced complex-cytomembrane interaction to control neuronal differentiation of eMSCs. During this process, atRAN release triggered by lysosomal acid activated MSC differentiation potential. Following stereotactic injection into hippocampus of AD model mice, miR-124 release was triggered by intracerebral ROS accumulation. eMSCs then underwent neuronal differentiation mediated by miR-124-induced neuron-specific programming activation, resulting in neural circuit reconstruction26.
