Table 1 Summary of the public datasets analysed in this study

Reference	Species	Tissues	Age range	No. of cells	Accession number	Protocol
Enge et al.³⁰	Human	Pancreas	1 mo–54 yrs	2544	GSE81547	Smart-seq2
Muraro et al.³¹	Human	Pancreas	23–59 yrs	12,200	GSE85241	CEL-seq2
Voigt et al.³²	Human	Retinal epithelium and choroid	54–92 yrs	8217	GSE135922	10x
Almanzar et al.³³	Mouse	Pancreas, liver	3–24 mo	9380	AWS	Smart-seq2
Corces et al.³⁴	Human	Brain tissue	38–95 yrs	4835	GSE147672	10x ATAC (single-nucleus)
Raredon et al.³⁵	Human	Lung	76–88 yrs	3630	GSE133747	10x (single-cell)
Raredon et al.³⁵	Mouse	Lung	9 wks	4221	GSE133747	10x (single-cell)
Raredon et al.³⁵	Rat	Lung	9 wks	4429	GSE133747	10x (single-cell)
Raredon et al.³⁵	Pig	Lung	3 mo	1746	GSE133747	10x (single-cell)
Buenrostro et al.⁴³	Human	Cell lines	N/A	1344	GSE65360	scATAC-seq
Ma et al.⁶⁵	Rat	Brown adipose tissue, liver	5–27 mo	29,165	GSE137869	10x (single-cell)
Smajić et al.⁶⁹	Human	Midbrain	66–93 yrs	41,435	GSE157783	10x (single-nucleus)
Grubman et al.⁹¹	Human	Entorhinal cortex	67–91 yrs	13,214	GSE138852	10x (single-nucleus)
Camunas-Soler et al.⁹²	Human	Pancreas	23–72 yrs	3789	GSE124742	Smart-seq2
				∑ = 140,435

Overall, we analyse sequencing data of more than 140,000 cells in four different species and seven different tissues, as well as cell line data.

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