Fig. 1: TaCLE24b positively contributes to lateral root development in wheat. | Nature Communications

Fig. 1: TaCLE24b positively contributes to lateral root development in wheat.

From: The TaCLE24b peptide signaling cascade modulates lateral root development and drought tolerance in wheat

Fig. 1

a RT-qPCR analysis shows the root-specific expression of TaCLE24b in wheat. The mean expression level of TaCLE24b in roots is set to 1.00. The box plots display the interquartile range, comprising the first quartile, median, and third quartile, while the whiskers extend from the minimum to the maximum values. Two-tailed Student’s t-test, (n = 9). b GUS staining shows the expression patterns of TaCLE24b in the lateral root tip, primary root tip, and lateral root primordium of wheat (n = 3). Scale bar, 1 mm. c Schematic analysis of three developmental zones of primary root regarding lateral root formation and outgrowth in wheat. The arrows indicate the magnified view of this region. d Phenotypic analysis of wheat seedlings in response to exogenous application of synthesized TaCLE24b. Fielder wheat seedlings were subjected to 9 day treatment with TaCLE24b at the 0.1 μM concentration before phenotyping. Scale bar, 5 cm. e Statistical analysis of wheat seedlings in response to exogenous application of 0.1 μM synthesized TaCLE24b. The box plots display the interquartile range, comprising the first quartile, median, and third quartile, while the whiskers extend from the minimum to the maximum values. Two-tailed Student’s t-test, (n = 30). DLRP: developing LRP; ELR: emerged LR; NELRP: non-emerged LRP. f Overexpressing TaCLE24b results in increased density of lateral root and lateral root primordium in CB037. Scale bar, 5 cm. Phenotyping was performed using 9 day-old seedlings of TaCLE24b overexpression lines (TaCLE24bOE-2 and TaCLE24bOE-3) and wild type (CB037) cultured in Hoagland medium. The box plots display the interquartile range, comprising the first quartile, median, and third quartile, while the whiskers extend from the minimum to the maximum values. Two-tailed Student’s t-test, (n = 30). g TaCLE24 knockout mutants show decreased density of lateral root and lateral root primordium compared to the control. Scale bar, 5 cm. Phenotyping was performed using 9 day-old seedlings of TaCLE24 knockout lines (Tacle24cr-3 and Tacle24cr-6) and wild type (Fielder) cultured in Hoagland medium. The box plots display the interquartile range, comprising the first quartile, median, and third quartile, while the whiskers extend from the minimum to the maximum values. Two-tailed Student’s t-test, (n = 30). h Comparison of lateral root density and lateral root primordium density of 9 day-old seedlings between Tacle24 knockout mutants (Tacle24cr-3 and Tacle24cr-6) and wild type (Fielder) in response to TaCLE24b peptide application. The box plots display the interquartile range, comprising the first quartile, median, and third quartile, while the whiskers extend from the minimum to the maximum values. Two-tailed Student’s t-test, (n = 30).

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