Table 2 Antibodies for western blot
Antibody | raised in | size (kDa) | Company | Cat # |
|---|---|---|---|---|
Pan 14-3-3 | Mouse | 30 | Santa Cruz | sc1657 |
Anti-Mouse HRP | Goat | - | Bio-Rad | 1706516 |
Anti-Rabbit HRP | Goat | - | Bio-Rad | 1706515 |
Phospho GCN2 Thr899 | Rabbit | 220 | CST | 94668 |
Total GCN2 | Rabbit | 220 | CST | 3302 |
Phospho mTOR Ser2448 | Rabbit | 289 | CST | 5536 |
Phospho AMPK Thr172 | Rabbit | 63 | CST | 2535 |
Phospho Akt Ser473 | Rabbit | 60 | CST | 4060 |
Phospho eIF2a Ser51 | Rabbit | 37 | CST | 3597 |
Total eIF2a | Rabbit | 37 | CST | 9722 |
Pan Akt1/2 | Rabbit | 60 | CST | 4691 |
NDUFA9 | Rabbit | 39 | In-house (Monash) | - |
EIF2AK1 (HRI) | Rabbit | 71 | MyBioSource | MBS2538144 |
OMA1 | Mouse | 40/60 | Santa Cruz | sc-515788 |
SDHA | Mouse | 71 | Abcam | ab14715 |
LC3A/B | Rabbit | 14/16 | CST | 12741 |
Ubiquitin | Rabbit | - | CST | 58395 |
Total AMPK | Rabbit | 62 | CST | 5831 |
