Fig. 3: PGC-1α transcript isoforms in the brain and cell type specificity.
From: Neuron-specific isoform of PGC-1α regulates neuronal metabolism and brain aging
A Schematic of the three major isoforms of Ppargc1a expressed in the brain and a representation of the location of their distinct promoter regions. B, C Relative expression pattern of PGC-1α transcripts detected by RT-qPCR of 12-month-old male mouse cortex (n = 6 mice) (B) and DIV10 primary cortical neurons and astrocytes isolated from P0 (neurons) and P1 (astrocytes) neonates (n = 6) (C). D Read density from GSE52564 for isolated neurons and astrocytes across the Ppargc1a locus. Pink lines denote annotated exons. E RT-qPCR detection of Ppargc1a transcript variants in cortex of male mice at 10 months, 20 months, and 30 months old (n = 5 mice). Boxplot represents median, 25th and 75th percentiles, and whiskers extend to min and max. F Expression of the brain-specific isoform of PGC-1α during neural stem cell (NSC) differentiation (n = 3 biological replicates). G Relative expression pattern of PGC-1α transcripts detected in DIV0 P0 neurons by RT-qPCR (n = 6 biological replicates). H Detection of Ppargc1a B1E2 during maturation of P0 primary cortical neurons (DIV0, 1, 3, 5, 7: n = 5; DIV10, 14: n = 6). I PGC-1α transcript levels after 24 h of actinomycin D treatment (n = 7). J Schematic of oxidation-reduction reaction of NAD+ to NADH. K Example of two-component decay curve produced through fluorescence lifetime imaging microscopy and is represented by the equation τm = a1(τ1)+a2(τ2). L Representative images (left) and distributions (right) of NAD(P)H mean fluorescence lifetime images of primary neurons and primary astrocytes (n = 10 neurons, n = 12 astrocytes). M Representative images (left) and quantitation (right) of NAD(P)H fluorescence intensity of primary neurons and primary astrocytes (n = 10 neurons, n = 12 astrocytes). Data shown with error bars noting mean ± SEM (B, C, F–I, M). Signficance determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons test (B, C, F–H), the Wilcox test (individual group comparison) and the Kruskal–Wallis test (E), or by two-way ANOVA with Sidak’s multiple comparisons test (I, M). Asterisks indicate p value of <0.05 (*), <0.01(**), <0.0001 (****). See Source data file for exact p-values. See methods section for details on biological replicates used for RT-qPCR.
