Fig. 3: Liver fibrosis impacts LV transduction of hepatocytes without major alteration of LV biodistribution.
From: Liver fibrosis negatively impacts in vivo gene transfer to murine hepatocytes
a Experimental design scheme (green arrows and bar indicate CCl4 administration or DDC diet, respectively) created with BioRender (https://BioRender.com/m06f740). LV.hFIX 5 × 1010 TU/kg. LV.mCherry in CCl4 experiment 1.5 × 1010 TU/kg, in DDC experiment 4 × 1010 TU/kg. b Individual values with mean of hFIX concentration. (Mann–Whitney test at the last time point). Gray, green, and orange symbols indicate mice treated with LV (n = 5), with CCl4 and LV (n = 5), or with DDC and LV (n = 4), respectively. c, d Individual values and mean ± SEM of percentage (%) of mCherry-positive hepatocytes (hepa; Mann–Whitney test). c Gray and purple symbols indicate mice treated with LV (n = 6) or with CCl4 and LV (n = 6), respectively. d Light blue and red symbols indicate mice treated with LV (n = 5) or DDC and LV (n = 5), respectively. e Individual values and mean ± SEM of VCN in indicated organs of mice administered with LV.hFIX (Mann–Whitney test). Gray, green, and orange symbols indicate mice treated with LV (n = 5), with CCl4 and LV (n = 5), or with DDC and LV (n = 4), respectively. f, g Individual values and mean ± SEM of VCN in indicated organs of mice administered with LV.mCherry (Mann–Whitney test). f Gray and purple symbols indicate mice treated with LV (n = 6) or with CCl4 and LV (n = 6), respectively. g light blue and red symbols indicate mice treated with LV (n = 5) or DDC and LV (n = 5), respectively. h Individual values and mean ± SEM of VCN in indicated liver cells of mice administered with LV.hFIX (Mann–Whitney test). Gray, green, and orange symbols indicate mice treated with LV (n = 5), with CCl4 and LV (n = 5), or with DDC and LV (n = 4), respectively. i, j Individual values and mean ± SEM of VCN in indicated liver cells of mice administered with LV.mCherry (Mann–Whitney test). i gray and purple symbols indicate mice treated with LV (n = 6) or with CCl4 and LV (n = 6), respectively. j light blue, and red symbols indicate mice treated with LV (n = 5) or DDC and LV (n = 5), respectively. Source data are provided as a Source Data file.
