Fig. 4: Milder fibrosis, induced by CCl4 or DDC, reduces LV and AAV vector transduction efficiency.
From: Liver fibrosis negatively impacts in vivo gene transfer to murine hepatocytes
a, b Representative histological images of liver sections from mice treated with CCl4 (n = 6) or fed with a DDC-supplemented diet (n = 8) for 3 weeks (a, bar = 600 µm, b bar = 300 µm). Picrosirius red staining. c Schematics of experimental design with timeline (green arrows indicate CCl4 administrations, green bar indicates DDC diet). LV.mCherry 1 × 1010 TU/kg. AAV.mCherry 1 × 1011 vg/kg. Created with BioRender (https://BioRender.com/t54x010). d, e Individual values and mean ± SEM of percentage (%) of the mCherry-positive liver area expressed over the total area analyzed (d Kruskal–Wallis test). d Gray symbols indicate mice treated with LV only (n = 6), green symbols indicate mice treated with CCl4 and LV (n = 8), and yellow symbols indicate mice treated with DDC and LV (n = 8). e orange symbols indicate female mice treated with AAV only (n = 4), red symbols indicate female mice treated with DDC and LV (n = 5), light blue symbols indicate male mice treated with AAV only (n = 5), blue symbols indicate male mice treated with DDC and LV (n = 5). f Individual values with mean ± SEM of mCherry-positive liver area percentage (%) expressed as fold on the mean of each respective control group (Mann–Whitney test; row data displayed in (e)). Brown symbols indicate mice treated with AAV only (n = 9), and purple symbols indicate mice treated with DDC and AAV (n = 10). Source data are provided as a Source Data file.
