Fig. 5: Liver fibrosis due to Abcb11 deficiency slightly reduces LV and AAV vector transduction, while LV and AAV vector transduction is not significantly impaired in Agl−/− mice.
From: Liver fibrosis negatively impacts in vivo gene transfer to murine hepatocytes
a, b Representative histological images of liver sections from 6-month-old Abcb11−/− mice (bar = 500 µm). Picrosirius red staining (a) and IHC CK19 (b). c, d Individual values and mean ± SEM of percentage (%) of the mCherry-positive liver area expressed over the total area analyzed (two-tailed Mann–Whitney test). LV.mCherry 3.5 × 1010 TU/kg. AAV.mCherry 1 × 1011 vg/kg. c Gray symbols indicate Abcb11+/+ mice treated with LV (n = 10), and orange symbols indicate Abcb1−/− mice treated with LV (n = 11). d brown symbols indicate Abcb11+/+ mice treated with AAV (n = 6), and blue symbols indicate Abcb1−/− mice treated with AAV (n = 5). e Representative histological images of liver sections from 9-month-old Agl−/− (left) and age-matched Agl+/+ male mice (right) (bar = 500 µm). Picrosirius red staining. f, g Individual values and mean ± SEM of percentage (%) of the mCherry-positive liver area expressed over the total area analyzed (Mann–Whitney test). LV.mCherry 5 × 1010 TU/kg. AAV.mCherry 1 × 1011 vg/kg. f Gray symbols indicate Agl+/+ mice treated with LV (n = 5), and orange symbols indicate Agl−/− mice treated with LV (n = 5). g Brown symbols indicate Agl+/+ mice treated with AAV (n = 5), and blue symbols indicate Agl−/− mice treated with AAV (n = 5). Source data are provided as a Source Data file.
