Fig. 2: APC/C ubiquitylates extranucleosomal histones in absence of canonical degron.
From: APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons
a APC/C-CDC20-mediated ubiquitylation assays using indicated nucleosomes, H3/H4 tetramers, or H2A/H2B dimers assembled with one fluorescently labeled histone indicated with asterisk. Cyclin BN* included as control APC/C substrate. Reactions were visualized by fluorescent scanning of SDS-PAGE gels. Representative images of n = 3 independent experiments. b Time course of H2B ubiquitylation by APC/C-CDC20 using H2A/H2B* dimer alone or bound to Nap1 histone chaperone as a substrate. Representative image of n = 4 independent experiments. c APC/C-dependent ubiquitylation assays with indicated substrates and HSL1 D-box and/or HSL1 KEN-box containing peptide competitors. Representative images of n = 2 independent experiments. d APC/C-mediated ubiquitylation assays with H3*/H4 tetramer substrates titrated with unlabeled H3/H4, Securin, or Cyclin BN competitor substrates. Representative image of n = 3 independent experiments. Source data are provided as a Source Data file.
