Fig. 4: Detection of cTnI in human serum spiked with cTnIC.
From: DNA origami signal amplification in lateral flow immunoassays
a A647 fluorescence assay. The left panel illustrates the 6HB signal amplification complex formed at the test line. The right panel shows test line fluorescence at 15 min assay time for Ab1-6HB with 100 A647-DNA labels and A647-labeled Ab1-DNA. Visual LoDs are highlighted. In the bottom-right panel, test line intensities are plotted against the cTnI concentration. Exposure times for fluorescence image acquisition are shown in the bottom-left corner of each panel. b Gold-DNA assay with Ab1-6HB, Ab2-6HB, and Ab2-gold. Test line images at 15 min are shown in the right panel, with visual LoDs highlighted. c Binding curves from panel (b), fitted with a 4-parameter logistic function. d Test line intensities near the LoD for Ab1-6HB. e LoD dependence on the assay time for Ab1-6HB. Panels d and e are displayed as mean ± SD (n = 3). Source data are provided as a Source Data file.
