Fig. 3: Cytoplasmic residues of ZorA form an extended pentameric rod.
From: Modularity of Zorya defense systems during phage inhibition
a Eight angstrom lowpass-filtered representative cryo-EM micrographs of purified ZorAB complexes (top) from Shewanella sp. strain ANA-3 (left) or Sulfuricurvum kujiense (right). Enlarged selected particles are depicted below the micrographs with their corresponding micrograph locations outlined as yellow boxes. Yellow arrowheads denote the cytoplasmic rods, and orange arrowheads denote the core complex embedded within the detergent micelle. Similar micrographs were obtained from three independent preparations of each sample. b (i) Two-dimensional averages of cryo-EM ZorAB particles from Shewanella sp. strain ANA-3 from focused classifications of the core (top) or rod structure (bottom). (ii) 2D averages of cryo-EM ZorAB particles from S. kujiense from focused classifications of the core (top) or partial core after particle subtraction (bottom). c Pentameric models of the ZorA cytoplasmic extensions based on Alphafold multimer predictions (residues 237–696 of Shewanella; left, residues 110–378 of Sulfuricurvum; right). Models colored by pLDDT score. d, Docking residues 111–140 of the S. kujiense ZorA cytoplasmic rod Alphafold model (residues 111–140) into a ~4.9 Å ZorAB cryo-EM volume containing partial helical rod density, with views shown from the side (left) or from the cytoplasm (right). e Full ZorAB models from type I (Shewanella sp. strain ANA-3; left) and type II (Sulfuricurvum kujiense) based on cryo-EM models of the ZorA5B2 core and Alphafold models of the cytoplasmic ZorA rod. Models in panels (e, f) were colored as in Fig. 1a, b.
