Fig. 1: Septin5 is abundant in pancreatic islet β-cells.

a Western blotting showing Septin5 is present in human and rodent islets. Rat insulinoma INS-1 is shown for comparison. b Western blotting showing Septin5 is present human islets, whose levels were not reduced in T2D human islets, as is also the case with GK rat islets in (a). SNARE proteins are reduced in both T2D human and GK islets (in (a)) compared to normal islets. c Quantification of band intensity (normalized to tubulin) in (b) showing similar expression level of Septin5 in human normal and T2D islets (n = 3 normal donors and 3 T2D donors). Statistical significance was assessed by a two-tailed Student’s t-test (n.s., not significant). For boxplots, boxes indicate 25th and 75th percentiles, the lines within boxes mark medians, whiskers above and below boxes indicate the 90th and 10th percentiles. d Lightning confocal images of human islet (top) and dispersed single β-cell (bottom) immunostained with Septin5 and insulin show Septin5 is abundant in pancreatic β-cells. A similar experiment was performed on mouse islets (Supplementary Fig. 1). e Quantification of endogenous Septin5 by immunostaining showing the heterogeneity of Septin5 expression in both dispersed human β-cells and intact islets from six non-diabetic and three T2D donors. Data are normalized to the average intensity. All the data shown are representative of at least 3 separate experiments. Source data are provided as a Source Data file.