Fig. 2: Energy-filtered MicroED data show improved statistics and high-quality maps.
From: Energy filtering enables macromolecular MicroED data at sub-atomic resolution
A Comparison of intensity statistics for filtered and unfiltered MicroED data, featuring from left to right: I/σI, CC1/2, and Rpim. B Slice through the structural model showing the electrostatic potential map, the location of the slice in the structure is indicated in the inset. C Maps and hydrogen omit maps are shown for Tyr128. D Maps and hydrogen omit maps for active site residues and waters. Electrostatic potential 2mFo–DFc maps are shown in blue and contoured at 4σ (B), and 2.2σ (C, D). Difference mFo–DFc maps are shown in green and red for positive and negative density contoured at 3σ (C, D). Hydrogen omit maps are shown in green and contoured at 2.3σ (C, D). Difference peaks marked with an asterisk indicate potential hydrogen atoms that have not been included in riding positions prior to map calculations.
