Fig. 6: The formation of ectopic additional cohesion sites is specific to meiosis.

a–c P. maniculatus cells (granulosa cells) arrested in mitosis by Nocodazole were fixed and stained for HEC1 together with Survivin (a), phosphorylated Aurora kinase (b), or H2A-pT121 (c). Immunostained cells were then labeled for PMsat using the Oligopaint technique. n = 13, 21, and 11 cells from three independent experiments were analyzed. Line scans of the signal intensities of Survivin (a), pAurora (b), or H2A-pT121 (c) together with PMsat and HEC1 were performed along the chromosome. Signal intensities of Survivin (a), pAurora (b), and H2A-pT121 (c) at PMsat were quantified; each dot represents one chromosome; n = 197, 147, and 128 chromosomes from three independent experiments were analyzed for Survivin, pAurora, and MCAK, respectively; unpaired two-tailed Mann-Whitney test was used to analyze statistical significance, ****P < 0.0001; red line, median. The images are maximum projections showing all the chromosomes (top) and optical sections to show individual chromosomes (bottom); asterisks denote the chromosomal location of internal PMsat (orange) and telomeric PMsat (yellow) on dual PMsat chromosomes; scale bars, 5 µm. d, Model for the centromere and pericentromere specification in mitosis and meiosis when a chromosome carries single or dual centromere satellite block. Source data are provided as a Source Data file.