Fig. 5: The combination of Lipin1 knockdown with Pten/Socs3 knockout and CNTF expression accelerates axon regeneration and functional recovery after pre-OPN OTI.

A Schematic diagram illustrating the experimental design for the combination of Lipin1 knockdown with Pten/Socs3 knockout and CNTF expression in the pre-OPN model. B Fluorescence images showing CTB-labeled RGC axons (green) and GFAP-labeled lesion site (red) in LGN and OPN from different groups. The dash lines outline the OPN regions. Scale bar: 200 µm. C Quantification of CTB-FITC intensities of labeled RGC axons at different distances from the lesion site. Data presents mean ± SEM. n = 7 (3 m Injury Ctrl.) and n = 9 (3 m PSCL Regen.) mice per group. Statistical significance: p < 0.0001 (100–200 µm), p = 0.0023, 0.0064, 0.0032 (300–500 µm). ANOVA followed by Bonferroni test, two-sided. D Quantification of percentages of pupil constrictions during PLR tests in different groups at different time points post-injury. Data presents mean ± SEM. n = 7 (3 m Injury Ctrl.) and n = 9 (3 m PSCL Regen.) mice per group. Statistical significance: p ≤ 0.0001, ANOVA followed by Bonferroni test, two-sided. E Schematic diagram showing the experimental design for chronic optic tract injury. F Quantification of percentages of pupil constrictions during PLR tests in different groups at different time points post-injury. Data presents mean ± SEM. Statistical significance: n = 5 (Injury Ctrl.) and n = 9 (chronic PSCL Regen.). p = 0.0042, 0.0026 (6 m, 7 m). ANOVA followed by Bonferroni test, two-sided. G Quantification of the CTB-FITC intensities of the RGC axons at varying distances from the lesion site. Data presents mean ± SEM. n = 5 (7 m Injury Ctrl.) and n = 9 (7 m chronic PSCL Regen.). Statistical significance: p < 0.0001 (100 µm), p = 0.0009 (200 µm), p = 0.013 (300 µm). ANOVA followed by Bonferroni test, two-sided. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 Source data are provided as a Source Data file.