Fig. 6: Neuronal and presynaptic modulation improves functional recovery.

A Schematic diagram showing the experimental design for PLR tests in PSCL Regen. mice with or without melanopsin overexpression (OE). B Quantification of percentages of pupil constrictions during PLR tests in different groups at different time points post-OTI. Data presents mean ± SEM. n = 12 mice. Statistical significance: p = 0.0076 (3 m), ANOVA followed by Bonferroni test, two-sided. C RNAseq results reveal the gradually decreasing expression of several key genes involved in presynaptic release machinery in mice receiving AAV-Cre+AAV-CNTF + AAV-shLipin1 (PSCL) over time after injury, compared to the control group receiving only AAV-GFP injection (Ctrl.). D Schematic diagram showing the experimental design for PLR tests with or without R-roscovitine and S-roscovitine intraperitoneal administration at 3 months post-injury. Two PLR tests in response to 0.5 mW/cm² blue light stimulation were conducted with a 1-day interval. Twenty minutes prior to the 2nd test, mice received intraperitoneal injections of either R-roscovitine or S-roscovitine. E Quantification of percentages of pupil constrictions in different groups with 0.5 mW/cm² light stimulation. Data presents mean ± SEM. n = 6 (Injury Ctrl.) and n = 10 (PSCL Regen.). Statistical significance: R-roscovitine: p > 0.999 (Injury Ctrl.), p = 0.0062 (PSCL Regen.), S-roscovitine: p > 0.999 (Injury Ctrl., and PSCL Regen.), ANOVA followed with Bonferroni test, two-sided. ns, not significant, p > 0.05, ** p ≤ 0.01. Source data are provided as a Source Data file.