Fig. 3: Raman spectroscopy of individual spongin fiber.
From: Discovery of mammalian collagens I and III within ancient poriferan biopolymer spongin
a Raman spectrum of spongin fiber with analyzed amide I band marked in green. b Deconvolution of amide I band in Raman spectra of spongin. The individual bands are marked in green, while the red band represents the sum of all the individual bands. c Amide I band of spongin in comparison to reference samples of Col I and Col III. Percentage amount of secondary structure of proteins in spongin (d), Col I (e), and Col III (f). Data are presented as mean values. The mean value was calculated from n = 165 data points, with error bars representing the standard deviation. g Camera image of the spongin fiber subjected to analysis. h Raman spectra measurement grid. i Percentage distribution of alpha helix, beta sheet, beta turn and random coil conformations in spongin. Source data are provided as a Source Data file.
